365 nm LED laser treatment on beagle for gingival whitening without gum dermabrasion

PURPOSE: Gingival whitening is one of dental treatment purposes which is close to treating aesthetic disorders. Initial gingival whitening treatment was done by dermabrasion using a high power Diode Laser. However, this treatment method cannot be free from any infection or pain after the treatment. Therefore, we have decided to progress gingival whitening treatment using a low power LED laser. MATERIALS AND METHODS: The laser was irradiated on pork meat then the safety of output power, temperature change and skin denaturalization was measured. Bison 365 nm LED laser was irradiated on oral mucosal pigment of a 15 - 20 kg beagle for 15 min for 1 - 2 weeks, one or two times each. Any pigment loss was checked through Hematoxyline-Eosin staining. RESULTS: The melanin pigments at the area of 365 nm LED Laser irradiation were decreased. CONCLUSION: The 365 nm LED Laser proposed in this study is considered to compensate the bleaching effect achieved by either using Diode laser or surgical methods.

Evaluation of ‘cattle’ and ‘Indian Bison’ type antigens of Mycobacterium avium subspecies paratuberculosis for diagnosis of Bovine Johne’s Disease using ‘indigenous ELISA’ and AGPT .

Two antigens (‘cattle’ type and ‘Indian Bison’ type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne’s disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.

Seroprevalence of hepatitis E virus in zoo animal species in Korea / 대한수의학회지

Hepatitis E virus (HEV) can infect not only human but also several animals. This study has been conducted to evaluate the comprehensive anti-HEV seroprevalence in zoo animals in Korea. Anti-HEV antibodies were identified in 14 of 64 zoo animal species. HEV antibodies were detected for the first time in Eurasian Lynx, Setland Pony, Fallow Deer, Ezo Sika, Formosa Deer, East Wapitis, Barasingha, Corriedale, American Bison, Guanacos, Reticulated Giraffe, and Saanen. These results indicate that the several zoo animal species were exposed to HEV.

A Single Thorax (Buffalo Chest) / 결핵

A-59-year old man was admitted to the hospital with a 2-month history of the right pleuritic chest discomfort and mild dyspnea. Seven years earlier, he was operated on left pneumonectomy for squamous cell carcinoma of left upper lobe (Stage IIb, T2N1M0). The computed tomographic scan revealed an anterior herniation of the right lung in the left hemithorax. A single thorax after receiving pneumonectomy is called a "buffalo chest" because the absence of an anatomical separation of the two hemithoraxes resembles that of the North American buffalo or bison. A possible pneumothorax should be catastrophic for the patient, so he should be closely monitored regarding any invasive procedure or trauma. He improved after symptomatic treatment.

Análise microbiológica do requeijão marajoara no norte do Brasil / Analyse of microbiological hand-made marajoara cheese in north of Brazil

Foram realizadas análises em quatro tipos de queijo, dois de leite de búfala e dois de vaca, sendo um de cada espécie feito de forma artesanal e outro com tecnologia. Os queijos artesanais foram do setor produtivo (ilha do Marajó), enquanto os outros elaborados no Laboratório de Agroindústria da Embrapa Amazônia Oriental, utilizando leite de rebanhos dos Campos Experimentais "Dr.Felisberto Camargo", em Belém, PA, e de Terra Alta, em Terra Alta, PA, cujos animais eram mantidos sob cuidados de higiene e sanidade. As avaliações microbiológicas foram iniciadas no dia subseqüente ao da fabricação e a intervalos de sete dias, durante aproximadamente um mês. Os dados foram analisados pelo programa estatístico NTIA. Não foi detectada a presença de S. aureus e Salmonellas, embora a presença de coliformes totais e fecais, bolores e leveduras comprovem contaminação microbiológica.

Avaliação microbiológica de iogurte de leite de búfala, com sabor de frutas da Amazônia, para merenda escolar / Microbiological evaluation of buffalo milk yogurt, with Amazônia fruits, for lunch school

Foram elaborados iogurtes de leite de búfala, com sabores de cupuaçu, bacuri e natural. Foram realizadas análises microbiológicas (coliformes totais e fecais, Salmonella, bolores e leveduras), as quais indicaram o iogurte como sendo de ótima qualidade e destacada aceitação. O iogurte de leite de búfala constitui um excelente produto para ser usado como alternativa em Programas Especiais financiadas pelo Governo, visando minimizar a fome de estudantes das redes públicas federal, estadual, municipal e filantrópica, além de comunidades de baixa renda, pois é um alimento nutritivo de elevado valor calórico-protéico e de baixo custo, além de usar matérias primas produzidas em nível local, gerando renda e trabalho através do incentivo à produção leiteira e à fruticultura regionais.

Histo-morphological study of ovarian follicles in relation to the presence of Corpora luta in buffalo - cow

This study aimed to investigate the relationship between histomorphological appearance of granulosa cells and oocytes recovered from follicles in relation to the presence of corpora lutea. The ovaries were collected at a local slaughterhouse from 22 buffalo-cows whose reproductive organs showed no macroscopic abnormality. Out of 35 visible follicles [>/10 mm in diameter] dissected, 24 follicles were on ovaries bearing corpora lutea [CLO] and 11 follicles were on ovaries non bearing corpora lutea [NCLO]. The follicular contents were separately aspirated and examined for the quality of oocyte recovered. The follicular walls were dissected and prepared for the light and electron microscopic examination. Fourteen oocytes were recovered from 35 follicles. Only 33.3% of oocytes recovered from follicles on CLO compared with 80% of oocytes recovered from follicles on follicles on NCLO classified as intact oocytes. Light microscopic observations of granulosa cells of these follicles [nineteen follicles on CLO and eight follicles on NCLO] showed that sixteen follicles on CLO and only one follicle on NCLO were atretic. Ultrastructural observation of granulosa cells revealed different degrees of cell affection in granulosa cells of follicles on CLO. This affection included dilatation and fragmentation of the rough endoplasmic reticulum together with the appearance of numerous lysosomes and fat droplets. The nuclei were electron dense, irregular and eccentric. Thus, it was concluded that most of visible follicles [>/ 10 mm in diameter] on ovaries bearing corpora lutea [middle of estrous cycle] showed many degenerative changes and most of oocytes recovered from these follicles were denuded